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See bacterial contamination on surfaces in real time We all know how a fluorescent light works, where an atom has energy put to it and one or more of the electrons orbiting the center of the atom become excited by the additional energy and jump up to a higher orbit. When the external energy is removed, the electron returns to it's original orbit and emits the energy in the form of light.

Now imagine this taking place within the phosphorescent compounds in bacteria. Just like a florescent light, they glow. Luminescence meters work on this principle, whereby a surface is sampled with a swab, the swab is inserted in a tube, and low intensity luminescence is induced chemically to react with ATP, a form of chemical energy found in all living things, including live bacteria. Light output is achieved.

The device measures the light and a unit of measurement is endeavored under the premise that more ATP equals more light equals more bacteria. As a sampling tool for surfaces, luminescence meters have some limitations. First, You have to sample a surface you cannot see, so it is uncertain what was sampled. Second, you have to hope that what you sampled with uncertainty does not have an invisible layer of biofilm that obscures the ATP signal of the bacteria.

Lastly, a leap of faith regarding the relationship between emitted light and a number on a readout intended to measure a quantity is required, and that number is based on several progressively dependant physical and chemical processes, all subject to variation. Many users claim they get erratic results and cannot explain why.

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Don’t get me wrong, the science is sound, but in practical application on surfaces in the wild, it just does not make the grade as a measuring instrument for industrial surfaces. So what would happen if we could detect that same type of luminescence without external chemicals or contact, on a surface? It would be ideal if the released energy was at or near the visible light spectrum so we could see it with our own eyes, in real time. Previous methods used cumbersome light sources, reflectors, refractors, filters, and complicated alignment procedures, but a new technology eliminates these barriers. It is now possible to see the contamination on large or irregular surfaces. Target species bacteria can be located on hard to reach areas without having to actually physically contact all of the surfaces first. If a biofilm exists, the energy is directed to it and through it, exposing the living bacteria beneath the biofilm in real time.